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. 1999 Aug 3;96(16):8943–8948. doi: 10.1073/pnas.96.16.8943

Figure 3.

Figure 3

Identification of PLE-like elements in other genes. (A) PLEs A and B of the albumin gene and the transferrin gene PLE are aligned with PLE-like sequences in mRNAs from several members of a family of related zinc finger transcription factors. See the text for the full names of each transcription factor. (B) PCR was performed on DNA extracted from Jurkat T cells to determine whether the PLE-like element identified in the HIV-EP2 gene is within the terminal exon. Primers were designed to encompass a 1,516-bp region of the cDNA sequence from the PLE-like element to a site 158 bp 5′ to the poly(A) addition site. The PCR products were separated on a 1% agarose gel and visualized by ethidium bromide staining. (C) Poly(A) tail length was determined for HIV-EP2 and IL-2 mRNAs in PMA-treated Jurkat T cells. Nuclear RNA was harvested from Jurkat T cells treated with phytohemagglutinin plus PMA for the indicated times. The products of the RT-PCR assay for poly(A) tail length were separated on a 6% polyacrylamide/urea gel and visualized by autoradiography. M, molecular size marker HinfI-digested OX174 DNA.