Skip to main content
. 1999 Aug 3;96(16):8949–8954. doi: 10.1073/pnas.96.16.8949

Figure 1.

Figure 1

Substrate and reaction paths for mutant lysozyme T26H. The substrate (Top) was produced by partial digest of E. coli cell walls with HEWL as described (14). R = dAla-dGlu-DAPA. The terminal NAM was reduced, as shown, to distinguish the respective NAG-NAM units produced by hydrolysis (Bottom Left). In addition, this reduction also makes it possible to determine whether the product shown bottom left is the α- or β-anomer. Transglycosylation (Right) can lead to a variety of products. For example, the condensation of a disaccharide of NAG-NAM (Bottom Left) with the original substrate (Top) leads to the hexasaccharide (n = 2) (Bottom Right).