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Comparison of the products formed by the digestion of the substrate shown in Fig. 1 with WT lysozyme and mutant T26H. (a) Substrate alone. (b) Digestion of 2 × 10−6 M substrate by 8 × 10−8 M WT* lysozyme for 5 min at 25°C (14). (c) Digestion of 2 × 10−6 M substrate by 5 × 10−7 M T26H for 5 min at 25°C (14). (d) Digestion of 8 × 10−5 M substrate by 5 × 10−7 M T26H for 60 min at 0°C. See text for additional details and explanation of peaks.
