Table 3.
Crystallographic data for mutant lysozymes
| Mutant | E11H | E11N | D20E | D20C | T26D | T26E (Apo) | T26H | T26Q |
|---|---|---|---|---|---|---|---|---|
| Cell dimensions, Å | ||||||||
| a, b | 61.1 | 61.1 | 61.0 | 61.0 | 60.9 | 61.2 | 61.5 | 61.1 |
| c | 96.8 | 96.8 | 97.0 | 97.1 | 96.9 | 96.3 | 97.7 | 96.3 |
| Total reflections | 40,327 | 33,301 | 53,978 | 28,398 | 39,326 | 30,082 | 105,345 | 33,225 |
| Independent reflections | 17,643 | 17,670 | 19,961 | 15,116 | 19,920 | 9,358 | 22,032 | 15,148 |
| Rmerge, % | 6.3 | 6.8 | 7.6 | 4.4 | 3.0 | 7.0 | 6.4 | 4.3 |
| Resolution used | 20–1.9 | 20–1.8 | 20–1.8 | 20–2.0 | 20–1.85 | 19–2.3 | 20–1.9 | 20–2.1 |
| Reflections included | 16,175 | 18,223 | 20,727 | 13,237 | 14,752 | 9,350 | 17,129 | 11,761 |
| Completeness, % | 92 | 88 | 95 | 87 | 78 | 96 | 95 | 89 |
| Δbond length, Å | 0.013 | 0.013 | 0.018 | 0.019 | 0.014 | 0.016 | 0.017 | 0.015 |
| Δbond angle, ° | 2.6 | 2.4 | 3.0 | 2.9 | 2.6 | 2.6 | 3.0 | 2.8 |
| Δplanes, Å | 0.010 | 0.011 | 0.014 | 0.015 | 0.012 | 0.017 | 0.014 | 0.012 |
| R, % | 16.5 | 16.5 | 16.7 | 16.5 | 15.5 | 18.4 | 16.9 | 16.6 |
WT* lysozyme crystallizes in space group P3221 with cell dimensions a = b = 61.2 Å, c = 96.8 Å (21). The crystals of all mutants are isomorphous. Rmerge gives the agreement between independently measured intensities while R is the crystallographic residual after refinement. Δbond lengths, Δbond angle, and Δplanes give, respectively, the average deviations of bond lengths, bond angles, and planar groups from their expected values. X-ray date also were measured for the double mutants E11H/T26H, D20C/T26D, and D20C/D26H. Preliminary analysis did not suggest anything unusual about these structures. They were not refined and are not included here.