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. 2002 Dec;86(12):1412–1416. doi: 10.1136/bjo.86.12.1412

Figure 3.

Figure 3

Degradation of mucins incubated with bacteria. Changes in the hydrodynamic volume of mucins were assessed by gel filtration after 16 hour incubation with bacteria. Mucins maintained in the same conditions, but without bacteria served as controls. The cross reactions: fractions(a) = (cross reaction(a)/Σ cross reactions) are presented as fractions of the total cross reaction of the eluent with the chosen reagent. Anti-M1 cross reaction after incubation with ocular surface isolate 21C. This cross reaction highlights proteolytic cleavage in MUC5AC mucins that occurs irrespective of mucin buoyant density. Vo mucin 1.35–1.4 g/ml after incubation with ocular surface isolates 3A and 21C: cross reaction with antiM1 and TKH2 to sialylTn. TKH2 negative MUC5AC mucins are obtained in each case. Contact lens adhering mucin buoyant density 1.3–1.4 g/ml incubated with contact lens derived isolates R37 and R30: cross reaction with antiM1. This mucin fraction was obtained after extraction with 10 mM DTT that solubilises aggregates.