Figure 5.

Ion exchange chromatography on MonoQ of contact lens extracted Vo mucins of buoyant density 1.35–1.4 g/ml after incubation with isolates from contact lenses worn by asymptomatic patients. First extraction mucins are soluble in GuHCl, second and third extractions were performed in the presence of DTT. (A) First extraction control mucins (M1, grey) and M1 after incubation with isolates R30 (solid symbols) and R37 (open symbols). (B) Second extraction control mucins (M2, grey circles) and M2 after incubation with isolate P30. (C) Third extraction control mucins (M3, grey circles) and M3 after incubation with isolates P30 (solid symbols) and R30 (open diamonds).