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Reduction of nuclear immunoreactivity of Smad4 in elongating lens cells. In (A) (case 9), some of the lens cells are positive for nuclear Smad4 (arrowheads), whereas others are negative for nuclear Smad4 (arrows). In (B) (case 7) and (C) (case 20), the cells with strong nuclear immunoreactivity tend to be cuboidal (arrowheads) whereas in regions where the cells appear to be elongating, Smad4 immunoreactivity tends to be absent or reduced (arrows). Indirect immunostaining. Bar, 20 μm.
