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. 2002 Dec;86(12):1428–1433. doi: 10.1136/bjo.86.12.1428

Figure 7.

Figure 7

Intracellular immunolocalisation of Smads3/4 in lens cells amid ECM on the healing capsules. (A) Lens cells embedded in ECM exhibit nuclear immunoreactivity for Smad3 (arrowheads) in specimens from case 9 (0.75 years postoperatively). (B) Lens cells with nuclei positive for Smad4 (arrowheads) amid extracellular matrix accumulated inside the capsule of the specimen (case 9). (C) Some lens cells within the ECM show Smad4 immunoreactivity in nuclei (arrowheads), while others exhibit only faint cytoplasmic immunoreactivity. Lens cells attached to the intraocular lens (IOL) optic tend to exhibit the strongest nuclear Smad4 staining (case 13). (D) Nuclei of lens cells in the ECM that has accumulated around the optic portion of an IOL still stain for Smad4 2.7 years after surgery (arrowheads, case 12). No nuclear Smad4 positive cells are seen in the ECM (asterisk) that is not attached to the lens optic. (E) Shows increased magnification of the lens cells in the boxed area in (D). Broken line in (C) indicates the anterior surface of the anterior capsule. Indirect immunostaining. Bar, 50 μm for (A) and (B) and 20 μm for (C) to (E).