Figure 3.

Interaction between HNF-6 and the GR. (A) Rat-1 fibroblasts were transiently transfected with a reporter containing the luciferase gene driven by a TATA box and six HNF-6-binding sites, in the presence or absence of expression vectors coding for HNF-6α or for the GR–VP16 and GAL4–VP16 fusion proteins as indicated. Representative experiments performed in duplicate are shown. (B) Bacterially expressed GST and the GST–HNF-6α and GST–GR fusion proteins were bound to glutathione-Sepharose beads; ¹⁴C-labeled GR or ¹⁴C-labeled HNF-6α produced in a wheat germ extract was incubated as indicated for 2 h at 4°C with the beads, which were then washed and processed for SDS/PAGE followed by autoradiography. An aliquot (1/10 of the input in the incubation mixture) of the radioactive proteins was run as a control (lanes 1 and 8).