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Effect of PKC inhibitor on ICAM-1 expression. HCE cells from the limbal explant were treated with 4 μmol of calphostin C, a specific PKC inhibitor, for 1 hour, followed by 2 days of incubation with modified SHEM and immunostaining with anti-ICAM-1 mAb. Immunoreactivity for ICAM-1 (brown stain) decreased markedly on calphostin C treated HCE cells (A) compared to that on non-treated HCE cells (B) in the leading zone. Original magnification, ×85.
