Table 2.

Testing the reading-frame dependence of the rpoH enhancer

Plasmid	Sequence	Activity
pDBZwt	AUG ACU CAC AAA AUG CAA AGU UUA GCU UUA GCC CCA AGU	1,448  ± 384
pDBZGA	AUG ACU GAC AAA AUA CAA AGU UUA GCU UUA GCC CCA AGU	7,688  ± 930
pDBZ+1	AUG ACU GGA CAA AAU ACA AAG UUU *GCU UUA GCC CCA AGU	7,558  ± 1,296
pDBZ+2	AUG ACU GAG ACA AAA UAC AAA GUU **GCU UUA GCC CCA AGU	5,986  ± 981
pDBZ+3	AUG ACU GGA GAC AAA AUA CAA AGU ***GCU UUA GCC CCA AGU	3,937  ± 788

Activity measurements are given in Miller units of β-galactosidase activity (19). The G → A substitution that increases the activity of the wild-type rpoH-lacZ fusion 5- to 10-fold is indicated in italics. Base insertions are indicated in bold. Positions of base deletions are indicated by asterisks (∗).