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. 1999 Aug 3;96(16):8979–8984. doi: 10.1073/pnas.96.16.8979

Figure 1.

Figure 1

SF UV-vis spectroscopy at 412 nm. E441Q R1 (75 μM), CDP (3.34 mM), dTTP (0.2 mM), and DTT (10 mM) in Tris buffer were mixed with an equal volume of R2 (75 μM) in the same buffer. Data (1,000 points) were acquired to 20 s.