Table 2.

p Values* for comparison of GFAP immunoreactivity† between different histopathological groups for the three regions analysed

Comparison groups§	Region¥	Immunolabelling intensity	Immunolabelling area
Drusen v YN	Inner retina	0.0036	0.002
	Outer retina	NS	NS
	Total	0.0022	0.0018
Drusen v AN	Inner retina	0.0298	0.0139
	Outer retina	NS	NS
	Total	0.0179	0.0104
GA v YN	Inner retina	NS	0.0397
	Outer retina	0.0573¶	0.0341
	Total	0.0397	0.0207
GA v AN	Inner retina	NS	NS
	Outer retina	0.0619¶	0.0368
	Total	0.0692¶	0.0449
GA v Drusen	Inner retina	NS	NS
	Outer retina	0.0359	0.0267
	Total	NS	NS
AN v YN	Inner retina	NS	NS
	Outer retina	NS	NS
	Total	NS	0.0383

*p Values obtained using the Student’s t test. NS = not significant (p>0.05) by both Student’s t test and ANOVA with Student-Newmann-Keuls post hoc test.

¶ = significant (p<0.05) by ANOVA with Student-Newmann-Keuls post hoc test.

†GFAP immunoreactivity assessed for two parameters: Immunolabelling intensity (total fluorescence intensity and area (sum of particle analyses of fields labelled).

§Comparisons made between AMD (drusen and GA, geographic atrophy) and controls (AN, aged normal and YN, young normal); also between AN and YN.

¥Three regions of GFAP expression: inner retina (within NFL/GCL); outer retina (OLM to IPL/INL); total = inner retina + outer retina.