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Genetic evidence for the role of HisZ in histidine biosynthesis. L. lactis strains differing by the presence (WT) or the absence (HisZ−) of HisZ were grown at 30°C on chemically defined medium (CDM) without histidine (−his) or supplemented with 0.01% histidine (+his) or histidinol (+hol). plcZ stands for a high-copy-number plasmid derivative of pJIM2279 (22) Emr (erythromycin resistance) and containing HisZ gene under control of a constitutive promoter.
