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Expression of GlcNAcα1→4Galβ→R by pcDNAI-α4GnT. COS-1 cells were cotransfected with pcDNAI-α4GnT (C and D) or pcDNAI (A and B) together with pRcCMV-leu, or transfected with pcDNA-α4GnT alone (E and F). Sixty hours after transfection, the cells were fixed and examined by incubation with HIK1083 antibody (B, D, and F) or anti-leukosialin antibody, 1G10 (PharMingen) (A, C, and E), followed by FITC-conjugated anti-mouse IgM (for HIK1083) or anti-mouse IgG (for 1G10). (Bar = 50 μm).
