Expression of GlcNAcα1→4Galβ→R structures and class III mucin in the human gastrointestinal mucosa and the gastric adenocarcinoma ASG cells stably transfected by α4GnT cDNA. Gastric fundic mucosa (A and B), gastric pyloric mucosa (C and D), and colonic mucosa (E and F) were stained with HIK1083 antibody (A, C, and E) or subjected to paradoxical Con A staining (B, D, and F). Similarly, AGS cells stably transfected with pcDNAI-α4GnT (G and H) or pcDNAI (I and J) were stained with HIK1083 antibody followed by FITC-conjugated anti-mouse IgM (G and I) or subjected to paradoxical Con A staining (H and J). (Bars in B, D, and F = 200 μm; bar in the inset of A = 20 μm; bar in J = 50 μm).