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Incorporation of 3H-GlcNAc from UDP-[3H] GlcNAc to various acceptors by a soluble chimeric α4GnT. The acceptor concentration was 1.0 mM, except for 0.7 mM for Galβ1→4GlcNAcβ1→6(Galβ1→3)GalNAcα→pNP. The incorporated radioactivity was shown relative to that obtained when Galβ1→4GlcNAcβ1→6(Galβ1→3)GalNAcα→pNP was used as an acceptor. SEs are shown by bars.
