Table 1.

List of monoclonal antibodies

Antibody (clone)	Specificity	Source
AE5	Cytokeratin 3	Serotec, Oxford, UK
K4.62	Cytokeratin 19	Sigma Immunochemicals
V9	Vimentin	Sigma Immunochemicals
Polyclonal	Connexin 43	Zymed Laboratories, SanFrancisco, USA
MIB1	Ki67	Dako, Cambridge, UK
7JUL	p63	Novocastra laboratories, Newcastle, UK
ICR10	Epidermal growth factor receptor	Serotec, Oxford, UK
NA1/34	CD 1a (Langerhans cells)	Dako, Cambridge, UK
Q BEND/10	CD 34	Novocastra laboratories, Newcastle, UK
DAK A3	Chromogranin	Dako, Cambridge, UK
Polyclonal	Synaptophysin	Dako, Cambridge, UK
HMB 45	Melanocytes	Dako, Cambridge, UK
2BH PD7/26	Leucocyte common antigen	Dako, Cambridge, UK

*Antibodies to chromogranin, synaptophysin, melanocytes, and the leucocyte common antigen were employed to detect any unexpected expansion of other indigenous cells at the limbus.

(α Enolase was a kind gift from Professor J D Zieske, Schepens Research Institute, Boston, MA, USA.)