Figure 2.

T cell fibroblast contact is necessary for lymphocyte mediated fibroblast death. Mitomycin C treated fibroblasts (0.4 mg/ml) were incubated alone (no T cells) or co-cultured with T cells (E:T = 20:1). T cell/fibroblast contact was permitted in one co-culture group but inhibited in the other by cell culture inserts. T cell co-culture induced a significant reduction in fibroblast number only when contact was permitted (p = 0.029). There was no significant change in fibroblast number when cell contact was inhibited (p = 0.12). The data show means (SD) derived from a representative of two separate experiments performed in quadruplicate.