Figure 1.

DDA of cDNAs derived from mRNAs from etiolated and illuminated maize leaves and Northern blot analyses of these mRNAs. (A) Part of a gel from a DDA done with primers T12MG and AP12 (GATCTAACCG) is shown. Total RNA preparations from 10-day-old etiolated maize leaves (D) and greening leaves illuminated with white light (W) or red light (R) for 8 hr or 24 hr were subjected to differential display reverse transcription-PCR. Band No. 40 represents the light-regulated cDNA segment of L29. (B) Confirmation of the differential expression pattern of L29. Twenty-five μg of total RNA from etiolated leaves (D) or greening leaves illuminated with blue light (B) or red light (R) for 8 hr or 24 hr was fractionated electrophoretically in a 1% formaldehyde agarose gel, transferred, and probed with [α-³²P]dCTP-labeled cDNA of reamplified fragment No. 40. A single band of about 0.8 kbp was detected in both blue light- and red light-treated samples but not in the etiolated samples. Equal loading was confirmed by 23S rRNA hybridization.