. 1999 Aug 3;96(16):9009–9014. doi: 10.1073/pnas.96.16.9009

Table 2.

Deletion and substitution mutations in the tra box abolish activation and repression by TraR

Plasmid^*	tra box^†	β-Galactosidase activity^‡
		Activation^§				Repression^¶
		TraR⁻		TraR⁺		TraR⁻		TraR⁺
		AAI⁻	AAI⁺	AAI⁻	AAI⁺	AAI⁻	AAI⁺	AAI⁻	AAI⁺
pZLb251/pPBL1	ATGTGCAGATCTGCACGT	1	1	11	392	89	97	86	5
pZLbm4-15/pLMB4-15	ATGCGCAGATCTGCGCGT	3	1	2	2	104	98	96	101
pZLb251-D/pPBL1-D	ATGTGCATGCACGT	1	1	1	1	232	198	221	187

Each plasmid pair represents the constructs used for the activation and repression assays, respectively.

^†

Underlined nucleotides in the tra box sequence identify the BglII site in its wild-type and deleted forms or the symmetrical base substitutions at the 4 and 15 positions.

^‡

Expressed as units per 10⁸ colony-forming units. All values are the averages of three independent experiments with variations <10%.

^§

Assessed in A. tumefaciens.

^¶

Assessed in E. coli DH5α.