Table 2.

 L-selectin detection and lymphocyte adhesion in subjects with diabetes and controls

Method	Controls	Diabetics	p v control
L-selectin on CD3+(FACS)	74.17% (6.51) (n = 17)	63.94% (11.34) (n = 36)	0.004
L-selectin mRNA (RT-PCR)	1.246 (0.7) (n = 7)	1.086 (0.303) (n = 13)	0.8
Serum L-selectin (ELISA)	828 ng/ml (177) (n = 12)	987 ng/ml (243) (n = 28)	0.06
Lymphocyte adhesion	100% (n = 7)	144% (65) (n = 9)	0.1

Results of surface L-selectin on CD3+ cells detected by flow cytometry (% positive cells), nuclear mRNA detected by RT-PCR (L-selectin: β actin ratio), shed L-selectin in the serum (ng/ml) detected by ELISA and lymphocyte adhesion to a HUVEC monolayer (% of control value, which has arbitrarily been set at 100%) are tabled for patients with diabetes and controls. Mean values (SD) are indicated.