Figure 3.
Glucose induced NF-κB activation and the effect of supplemental IL-1β: western blots were performed for p65 subunit of NF-κB using antibody from Santa Cruz in the cells incubated with or without 10 ng/ml IL-1β. The band intensities were adjusted to the expression of the intrinsic protein, GAPDH in each sample. The blots are representative of three different cell preparations. *p<0.05 and **p<0.05 compared with 5 mM and 20 mM glucose respectively.