Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
15N HSQC spectra of 15N-labeled Elc1. (A) Free. (B) VHL(157–171)-bound. (C) Ela1(1–143)-bound. (D) Ela1(3–17)-bound. Peaks assigned are labeled. Samples A, B, and D are 0.7–1.5 mM in 10 mM NaPi, pH 7.0/100 mM NaCl/10 μM ZnSO4/100 μM EDTA/7.5 mM DTT in 90% H2O/10% D2O. Sample C is 0.7 mM in similar buffer but at pH 7.5 and 500 mM NaCl. Experiments were run at 30°C (A, B, and D) or 25°C (C).
