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Anion exchange elution profile of 20-mer scFv. Stepwise elution separated monomeric scFv, dimeric scFv and contaminants. The 50 mM NaCl step eluted three peaks containing monomeric scFv. The 70 mM NaCl step eluted dimeric scFv and 1 M NaCl eluted scFv complexed with contaminant protein.
