Table 2.

 Penetration of antibody fragments through pig corneas mounted in a corneal perfusion chamber

Antibody fragment	Molecular weight (kDa)	Protein concentration in eye drop (mg/ml)	Time until detectable penetration (h)	Protein concentration in chamber at 10 hours (µg/ml)
ScFv 20-mer (monomer)	27	1.0	7	0.11
		1.0	7	0.11
		1.1	6	0.50
		1.1	7	0.40
ScFv 20-mer (dimer)	56	0.8	7	0.39
		0.8	5	0.80
		0.8	7	0.36
		0.8	7	0.13
		0.8	6	0.18
ScFv 11-mer (dimer)	52	0.9	8	0.04
		0.9	7	0.09
		0.9	6	0.48
		0.9	6	0.47
Fab fragment (monomer)	48	2.3	6	0.08
		2.3	7	0.03
		5.2	7	0.25
		5.2	7	0.35
		5.2	7	0.45
Monoclonal antibody	∼150	Activity detectable at 1/30 000 dilution	Not detected	Not detected