Figure 2.

 Lactate dehydrogenase release assay showing (A) fibroblast apoptosis and (B) viable fibroblast number 7 days after 5 minute applications of 5-fluorouracil (25 mg/ml) (5-FU), mitomycin C (0.4 mg/ml) (MMC), or RPMI culture medium (control) with or without trypan blue (TB). Addition of trypan blue had no effect on MMC induced apoptosis or viable cell number for any treatment group. Data shown are mean (standard deviation) values derived from a representative of two separate experiments performed in quadruplicate.