Triamcinolone acetonide measured by high pressure liquid chromatography. Trigon depot, triamcinolone acetonide concentration in the original resuspended Trigon ampule; 0.22 μm Millipore and 5 μm Pall, triamcinolone acetonide concentration after the corresponding filter techniques; Sed 0.5 ml and Sed 0.9 ml, triamcinolone acetonide concentration after the corresponding sedimentation technique and pellet reconstitution with 0.9 or 0.5 ml of BBS; Cent 1 ml, triamcinolone concentration after the corresponding centrifugation technique. Bars are means of three independent assays performed in duplicate. Errors bars represent standard deviations. *Triamcinolone acetonide concentration statistically significantly higher than Trigon depot, **Statistically significantly lower than Trigon depot (p<0.05).