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. 2005 Dec;89(12):1638–1642. doi: 10.1136/bjo.2005.069591

Figure 3.

Figure 3

 Regulation of bovine gremlin expression. (A) RT-PCR analyses of quantitatively standardised total RNA samples from BRPC cultured in 5 mM glucose, 5 mM glucose and 25 mM mannitol (as an osmolarity control), 30 mM glucose or 30 mM glucose plus 10 μM PD 98059, or 10 μM GF 109203X, or 500 nM CCCP, or 1 μg/ml αTGFβ1 antibody for 7 days. 18S ribosomal RNA was used as a control for equal input amounts of RNA. (B) RT-PCR analyses of quantitatively standardised total RNA samples from BRPC cultured in 5 mM glucose (control), 5 mM glucose and 10 ng/ml TGFβ1, and 5 mM glucose and 10 ng/ml TGFβ2. 18S ribosomal RNA was used as a control for equal input amounts of RNA.