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. 1999 Aug 3;96(16):9051–9056. doi: 10.1073/pnas.96.16.9051

Figure 2.

Figure 2

Genetic organization and nucleotide sequence of the rifF gene in A. mediterranei S699 and HGF019. (A) A restriction map of the region of the A. mediterranei S699 chromosome encompassing the C-terminal end of the polyketide synthase (rifE) and the whole ORFs of the amide synthase (rifF), amino DHQ synthase (rifG), amino DAHP synthase (rifH), amino quinate/shikimate dehydrogenase (rifI), AHBA synthase (rifK), and oxidoreductase (rifL) genes (12). The three indicated NotI DNA fragments, 0.67, 1.9, and 2.5 kb, respectively, were used as a 32P-dCTP-labeled probe for the blot in B. (B) The autoradiograph of a Southern blot to verify the deletion of a 369-bp DNA fragment of rifF in A. mediterranei HGF019. A 0.8% agarose gel in which 5 μg of NotI-digested genomic DNA from strains S699 and HGF019 had been separated was blotted and hybridized with the three NotI DNA fragments shown above. The band corresponding to the deleted DNA fragment, 1.55 kb, of rifF in strain HGF019 is indicated by an arrow. (C) The nucleotide and deduced amino acid sequences of the rifF gene. The deleted 369-bp DNA fragment in strain HGF019 is underlined.