Skip to main content
. Author manuscript; available in PMC: 2007 Jan 17.
Published in final edited form as: Ocul Surf. 2004 Oct;2(4):229–247. doi: 10.1016/s1542-0124(12)70111-8

Figure 4.

Figure 4

Expression of β-defensins in regenerating corneal epithelium. The corneal epithelium (“original”) was scraped from cadaver corneas, then the corneas were placed in to organ culture and regenerated epithelial cells (“regrown”) collected 24 or 48 hours later. RT-PCR (A) and immunoblotting (B) were used to study defensin expression in the samples. A: M = size marker; Lanes 1,4,7,10 are products for the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase; Lanes 2,5,8,11. are products for hBD-1; Lanes 3,6,9,12 are products for hBD-2.B : Control are samples of synthetic defensin peptide (25ng/dot). hBD-1 is constitutively expressed but hBD-2 is only present in regrown samples. (Reprinted from McDermott AM, Redfern RL, Zhang B. Human β-defensin 2 is upregulated during re-epithelialization of the cornea. Curr Eye Res 2001;22:64-7, with permission of Current Eye Research.)