. Author manuscript; available in PMC: 2007 Nov 1.

Published in final edited form as: Arch Biochem Biophys. 2006 Sep 25;455(1):61–67. doi: 10.1016/j.abb.2006.08.024

Table 3.

Steady state kinetic analysis of 7-EFC O-deethylation by P450 2B4dH/H226Y and mutants.

P450	k_cat (min^-1)	K_m (μM)	k_cat/K_m
2B4dH/H226Y	9.9 (0.7)^a	108 (14)	0.09
V292A	11 (0.7)	102 (12)	0.11
F296A	5.0 (0.6)	73.3 (18)	0.07
T302A	1.3 (0.1)	67.8 (10)	0.02
I363A	5.3 (0.6)	81.8 (18)	0.07
V367A	1.8 (0.1)	284 (90)	0.01
V367L	8.3 (0.3)	67.0 (5.6)	0.12
V477A	4.7 (0.3)	85.7 (9.2)	0.05
V477F	9.5 (0.3)	42.6 (3.2)	0.22

Results are the representative of at least two independent determinations. The variation between the experiments is ≤ 20%.

Standard errors for fit to Michaelis-Menten are shown in parenthesis.