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Expressed wild type and constitutively active Rab11 recruits endogenous Evi5 to Rab11-positive endosomes. U2OS cells were transfected with GFP–Rab11 wild type or inactive S25N mutant, fixed with 4% PFA, and stained with anti-Evi5 antibody as well as Hoechst to mark the DNA. White arrows highlight sample regions of colocalization, which appear yellow in the merged panel.
