Fig. 3.

The islets of class I APC-bald NOD female mice have noninvasive insulitis lesions. (A and F) Hematoxylin and eosin stain of pancreata from >300-day-old, nondiabetic, class I APC-bald NOD mice showed infiltrates limited to the periislet region. Pancreatic sections were stained by immunofluorescence with anti-insulin antibody (red) and anti-CD8 antibody (green). Sections were additionally costained with anti-CD4 (B and G), anti-B220 (C and H), anti-CD11c (D and I), or anti-CD11b (E and J). The noninvasive infiltrate of pancreata from class I APC-bald NOD mice contained CD4 T cells, B cells, DCs, and macrophages, but it lacked CD8 T cells.