Figure 7.

Effect of tumour necrosis factor α (TNF-α) on basal and histamine induced levels of ³²P-phosphatidic acid (PA) (A) and ³²P-phosphatidylbutanol (PBut) (B). Cells were exposed to 10 ng/ml TNF-α over the 24 hour labelling period. Cells were stimulated with 100 μM histamine (hist). Application of histamine resulted in significantly increased levels of ³²P-PA and ³²P-PBut, as indicated in fig 5 ▶. Data are shown as percentage change from control experiments. Data are presented as means (SEM) of five monolayers. Statistical significance was evaluated using the Student's t test: *p<0.05.