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Pretreatment regimens and primary antibody concentrations for immunohistochemistry
| Primary antibody | Pretreatment | Dilution |
| CD3 (monoclonal) | Pressure cooker | 1:200 |
| CD20 (L26) | No treatment | 1:1000 |
| CD68 (PGM-1) | Microwave | 1:200 |
| MIB-1 | Microwave | 1:400 |
| Bcl-2 | Pressure cooker | 1:200 |
| Bax | None | 1:50 |
| Fas | Microwave | 1:50 |
| Fas ligand | Pressure cooker | 1:80 |
| Retinoblastoma protein | Microwave | 1:200 |
| TIMP-1 | Pronase | 1:200 |
| TIMP-2 | Pronase | 1:400 |
TIMP, tissue inhibitor of metalloproteinase.
