Figure 3.

Cdx2 promoter analysis for butyrate (NaB) stimulation. Caco2 cells were transfected with the pCdx2-1Luc reporter plasmid containing the promoter fragment between nucleotides −907 to +117 or with the shorter promoter fragments extending to positions −693, −551, −392, −338, or −171. The arrow indicates the transcription start site. pCMV-βGal was cotransfected for normalisation. Cells were then treated for 48 hours with 1 mM NaB or vehicle alone. Luciferase activity was related to β-galactosidase and the results are expressed as fold induction compared with control cells treated with vehicle alone. The results are means of three independent experiments.