Figure 3.

Afa/Dr DAEC C1845 induced F-actin rearrangements in human enterocyte-like Caco-2/TC7 cells develop as a function of the level of infecting bacteria. Confluent differentiated Caco-2/TC7 cells were infected apically at 37°C in a 10% CO₂/90% air atmosphere for three hours with C1845 bacteria. Fixed and permeabilised cells were processed for direct immunofluorescence labelling of F-actin with fluorescein labelled phalloidin, and relative immunofluorescence intensity (arbitrary units) was measured as described in materials and methods. (A) Quantification of the F-actin rearrangements as a function of the level of infecting bacteria. (B) Determination of the level of adhering C1845 bacteria as a function of the level of infecting bacteria.