Table 3.

Effect of LB-SCS treatment at a concentration which did not affect bacterial viability or adhesion on C1845 induced rearrangements of brush border associated structural and functional proteins in enterocyte-like Caco-2/TC7 cells

Assay	Control uninfected	C1845 infected	C1845 infected LB-SCS treated
F-actin
RI	2.78 (0.18)	0.32 (0.09)**	2.70 (0.20)*
SI
RI	2.78 (0.32)	0.40 (0.14)**	2.71 (0.25)*
Band (μm)	1.4–1.7	4.9–5.8	1.5–2.0
AP
RI	2.44 (0.21)	1.21 (0.15)**	2.39 (0.22)*
Band (μm)	1.5–1.8	4.5–5.7	1.6–2.2
DPP IV
RI	2.84 (0.25)	0.42 (0.21)**	2.78 (0.28)*
Band (μm)	1.8–2.0	4.3–4.7	1.8–2.1
GLUT5
RI	2.30 (0.17)	1.07 (0.15)**	2.07 (0.25)*
Band (μm)	1.6–2.0	5.3–6.7	1.7–2.2

RI, relative immunofluorescence intensity expressed as arbitrary units, measured at the apical domain by conventional immunofluorescence microscopy in unpermeabilised cells.

Band, size of the immunofluorescent band (μm) measured by confocal laser scanning microscopy in permeabilised cells (vertical x– z optical section).

SI, AP, DPPIV, GLUT5, sucrase-isomaltase, alkaline phosphatase, dipeptidylpeptidase IV, and fructose transporter, respectively; LB-SCS, spent culture supernatant of Lactobacillusacidophilus LB culture.

Data are mean (SEM) from three experiments.

Statistical analysis was performed using a Student's t test: *not significant, **p<0.01 compared with control uninfected cells.