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Hepatocyte couplets accumulating cholyl-lysyl-fluorescein (CLF) in the technique of canalicular vacuolar accumulation (cVA) of CLF. (A) Couplet preparation after 15 minutes of incubation with CLF. (B) Couplets exposed to 2.5 μM taurolithocholate (TLC); subsequently incubated with CLF for 15 minutes. (C) Couplets coincubated with TLC and tauroursodeoxycholate (TUDCA) (2 μM); subsequently incubated with CLF for 15 minutes.
