Figure 3.
Effect of inhibitors of protein kinase C (PKC), protein kinase A (PKA), and Ca2+ dependent signalling pathways on hepatoprotection induced by tauroursodeoxycholate (TU) against taurolithocholate (TLC) induced decrease in canalicular vacuolar accumulation (cVA) of cholyl-lysyl-fluorescein (CLF). Couplets were incubated for 15 minutes at 37°C with dimethyl sulphoxide (DMSO, control), 1,2-bis-(o-aminophenoxy)-ethene-N,N,N`,N`-tetra-acetate tetra-(acetomethyl)ester (BAPTA/AM (B)), H7, staurosporine (S), or KT5720 (KT). Then, TLC was added to dishes containing DMSO, and TLC+TU were added to the remaining dishes, and the cells were incubated for another 30 minutes. cVA of CLF was then analysed, as described in the methods section. Values are means (SD) (n=5–6). The protective effect of TU on TLC induced cholestasis was significantly decreased by the presence of B, H7, or S, but not by KT. Significantly different from TLC, ***p<0.001; significantly different from TLC+TU, ††p<0.01, †††p<0.001; NS, not significant.