Table 2.
Effect of the inhibitors of PKC, PKA, and Ca2+ dependent signalling pathways used in this study on cVA of CLF
| Control | BAPTA/AM | H7 | SP | KT5720 | |
| cVA of CLF (%) | 61 (7) | 57 (3) | 56 (7) | 55 (3) | 58 (10) |
Hepatocyte couplets were incubated for 30 minutes at 37°C with DMSO (control), BAPTA/AM, H7, SP, or KT5720, before adding CLF and assessing cVA of CLF, as described in the methods section.
All values are mean (SD); n= 4–6 separated set of couplet preparations.
Differences between groups were not statistically significant.
CLF, cholyl-lysyl-fluorescein; BAPTA/AM, 1,2-bis-(o-aminophenoxy) -ethene-N,N,N`,N`-tetra-acetate tetra-(acetomethyl)ester; DMSO, dimethyl sulphoxide; cVA, canalicular vacuolar accumulation; PKA, protein kinase A; PKC, protein kinase C; SP, staurosporine.