Table 3.

Effect of the inhibitors of PKC, PKA, and Ca²⁺dependent signalling pathways used in this study on TLC induced decrease in cVA of CLF

	TLC	TLC+ BAPTA/AM	TLC+H7	TLC+SP	TLC+KT5720
cVA of CLF (%)	25 (7)	24 (14)	22 (5)	28 (3)	22 (6.3

Couplets were incubated for 15 minutes at 37°C with DMSO (control), BAPTA/AM, H7, SP, or KT5720. Then, TLC was added to the dishes containing DMSO, and TLC was added to the remaining dishes, and cells were incubated for another 30 minutes. cVA of CLF was then analysed, as described in the methods section.

All values are mean (SD); n= 5–6 separated set of couplet preparations.

Differences between groups were not statistically significant.

CLF, cholyl-lysyl-fluorescein; BAPTA/AM, 1,2-bis-(o-aminophenoxy) -ethene-N,N,N`,N`-tetra-acetate tetra-(acetomethyl)ester; DMSO, dimethyl sulphoxide; cVA, canalicular vacuolar accumulation; PKA, protein kinase A; PKC, protein kinase C; SP, staurosporine; TLC, taurolithocholate.