Figure 3.

Extracellular signal regulated kinase (ERK) 1/2 activation in platelet derived growth factor (PDGF) stimulated pancreatic stellate cells (PSCs). PSCs of the second passage were starved from serum for 12 hours before they were stimulated with PDGF-BB (10 ng/ml) for the indicated periods of time. After cell lysis and immunoprecipitation of ERK 1/2, in vitro kinase assays (n=6 separate samples) were performed as described in materials and methods. The stimulation index corresponds to the ratio of ERK activities of PDGF stimulated and non-stimulated cells. *p<0.05 or higher degree of significance versus without PDGF.