Figure 2.

USF factors activate a TFF2-luc reporter gene construct. (A) The gastrointestinal cell line MKN45 was cotransfected with the TFF2-luc reporter gene construct and indicated amounts of expression plasmids encoding USF1, USF2, USF1/USF2, or c-Myc. (B) As in (A) but the cell line LS174T was used. (C) MKN45 cells were cotransfected with wild type (wt) or E box mutated (mut) TFF2-luc, with (+) or without (-) expression plasmids encoding USF1 or a mutant USF protein that cannot bind DNA (m). (D) As in (A) but the M4-mintk-luc reporter gene construct with four high affinity Myc/Max binding sites was used. (E) As in B but with the M4-mintk-luc reporter gene.