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. 2002 Nov;51(5):685–690. doi: 10.1136/gut.51.5.685

Figure 4.

Figure 4

Binding of USF, c-Myc/Max, and Max/Max complexes to TFF derived E box sequences. (A) Binding of COS-7 derived USF1/USF1 homodimers to radiolabelled CMD was competed with a 3-fold, 10-fold, 30-fold, or 100-fold excess of unlabelled CMD, or oligonucleotides spanning the E boxes of either TFF1, TFF2, or TFF3 as indicated. Supershift experiments were performed with an antibody specific for USF1 (α-USF1). Control: extract of mock transfected cells was used. * Non-specific complex. (B) Binding of COS-7 derived c-Myc/Max and Max/Max complexes were analysed as in (A) Supershift experiments were done with specific antibodies to c-Myc or Max (α-Myc or α-Max, respectively). The control is as in (A). * Non-specific complex.