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. 2002 Nov;51(5):685–690. doi: 10.1136/gut.51.5.685

Figure 5.

Figure 5

Binding of USF to the TFF2 promoter in cells. (A) ChIP analyses were performed on lysates of formaldehyde crosslinked HT-29 and MKN45 cells. One of 20 of the purified DNA from individual immunoprecipitations was subjected to PCR analysis (34 cycles). Fourfold dilution series of purified input DNA served as normalisation controls for the PCR reactions. (B) Quantification of ChIP reactions from HT-29 cells. Product intensities of samples and adequate twofold dilution series of purified input DNA were compared. Relative DNA binding defines the amount of PCR product obtained from immunoprecipitates compared with total input DNA with the amount of PCR product equivalent to 80 pg arbitrarily set as 1. The graph shows the mean value of two independent determinations. Insert: Lower amounts of input DNA were used to determine c-Myc and c-Myb binding equivalents (39 cycles). (C) ChIP analyses were performed as described under (A).