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. 2003 Jan;52(1):65–70. doi: 10.1136/gut.52.1.65

Figure 3.

Figure 3

Cellular expression of interleukin 17 (IL-17) in Crohn’s disease (A–F) and ulcerative colitis (G–L) patients. Two colour immunofluorescence was used to determine IL-17 expression (rhodamine red, red fluorescence: B, E, H, and K), CD3 expression (fluorescein isothiocyanate (FITC), green fluorescence: A, G), and CD68 expression (FITC, green fluorescence: D, J). Cells showing coexpression of IL-17 and CD3 (C, I) or IL-17 and CD68 (F, L) were visualised by a yellow colour. The specificity of each antibody was confirmed by negative reactivity of subclass matched normal IgG for anti-CD3 (M), anti-IL-17 (N), and anti-CD68 (O).