Figure 4.

Reverse transcription-polymerase chain reaction (RT-PCR) analysis of interleukin 17 (IL-17) mRNA expression. Purified T cells were stimulated by interleukin 2 for three hours, and purified monocytes/macrophages were stimulated by lipopolysaccharide for three hours. Total cellular RNA was extracted by the acid guanidium thiocyanate-phenol-chloroform (AGPC) method and IL-17 mRNA expression was evaluated by RT-PCR analysis. Similarly, IL-17 mRNA expression was investigated in mucosal samples from normal individuals and in active Crohn’s disease (CD) and ulcerative colitis (UC) patients.