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Immortalised oesophageal squamous cells grown on feeder layers retain p16 protein that can respond to cellular stresses. Western analysis shows that telomerase expressing oesophageal squamous cells grown on feeder layers and infected with RAS (+RAS) increased p16 protein levels by the third passage (P3) after RAS infection compared with uninfected cells grown on feeder layers (UI). HeLa cells served as the positive control for p16 expression.
